Enhanced determination of fertility hormones in saliva at disposable immunosensing platforms using a custom designed field-portable dual potentiostat

  • V. Serafín /
  • B. Arévalo /
  • G. Martínez-García /
  • J. Aznar-Poveda /
  • J. A. Lopez-Pastor /
  • J. F. Beltrán-Sánchez /
  • A. J. Garcia-Sanchez /
  • J. Garcia-Haro /
  • S. Campuzano /
  • P. Yáñez-Sedeño /
  • J. M. Pingarrón
Journal ar
Sensors and Actuators, B: Chemical
  • Volumen: 299
  • Fecha: 15 November 2019
  • ISSN: 09254005
  • Source Type: Journal
  • DOI: 10.1016/j.snb.2019.126934
  • Document Type: Article
  • Publisher: Elsevier B.V.
© 2019 Elsevier B.V.This work describes a new electroanalytical device for the simultaneous and reliable determination of two fertility relevant hormones (luteinizing hormone, LH, and progesterone, P4) in saliva. The device is constructed using a custom designed and field-portable potentiostat where dual disposable immunosensing platform are connected. The immunosensors are based on sandwich-type and competitive immunoassays implemented onto magnetic microbeads (MBs) functionalized with Neutravidin and Protein G for the determination of LH and P4, respectively. Amperometric detection performed at ¿0.20 V vs the Ag pseudo-reference electrode using the H2O2/hydroquinone (HQ) system was employed as the transduction technique after placing the MBs with immunocomplexes for each target hormone on the appropriate working electrode of screen-printed dual carbon electrodes (SPdCEs). The method exhibits high sensitivity and selectivity for the target hormones providing detection limits of 1.7 pg mL¿1 and 0.10 mIU mL¿1 for P4 and LH, respectively, with a 1 h test time. The applicability of the method was confirmed by determining both hormones in saliva samples from different volunteers providing results comparable to those obtained using amperometric transduction with a commercial potentiostat and with ELISA methodologies involving the same immunoreagents for each target hormone.

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