Plants as biofactories: Physiological role of reactive oxygen species on the accumulation of phenolic antioxidants in carrot tissue under wounding and hyperoxia stress

  • Daniel A. Jacobo-Velázquez /
  • Ginés B. Martínez-Hernández /
  • Silvia Del C. Rodríguez /
  • Cong Mei Cao /
  • Luis Cisneros-Zevallos
Journal ar
Journal of Agricultural and Food Chemistry
  • Volumen: 59
  • Número: 12
  • Fecha: 22 June 2011
  • Páginas: 6583-6593
  • ISSN: 00218561 15205118
  • Source Type: Journal
  • DOI: 10.1021/jf2006529
  • Document Type: Article
Plants subjected to postharvest abiotic stresses synthesize secondary metabolites with health-promoting properties. Here, we report the potential use of carrots (Daucus carota) as biofactories of caffeoylquinic acids when subjected to wounding and hyperoxia stresses. Wounding stress induced an increase of ~287% in total phenolic content (PC) in carrots stored for 48 h at 20 °C. This increase was higher (~349%) in the wounded tissue treated with hyperoxia stress. To further understand the physiological role of reactive oxygen species (ROS) as a signaling molecule for the stress-induced accumulation of phenolics in carrots, the respiration rate as well as the enzymatic activities of NADPH oxidase, superoxide dismutase, ascorbate peroxidase, and catalase were evaluated. Likewise, shredded carrots were treated with diphenyleneiodonium chloride solution to block NADPH oxidase ROS productions, and the phenylalanine ammonia lyase activity and total PC were evaluated. Results demonstrated that ROS play a key role as a signaling molecule for the stress-induced accumulation of PC in carrots. © 2011 American Chemical Society.

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